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Annotation and validation of genes involved in photosynthesis and starch synthesis from a
Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG
《农业科学与工程前沿(英文)》 2016年 第3卷 第4期 页码 308-320 doi: 10.15302/J-FASE-2016113
关键词: Manihot esculenta expressed sequence tag unigene pathway expression pattern
null
《医学前沿(英文)》 2017年 第11卷 第3期 页码 378-385 doi: 10.1007/s11684-017-0541-8
The purpose of this study is to characterize a meta-signature of differentially expressed mRNA in chronic kidney disease (CKD) to predict putative microRNA (miRNA) in CKD–mineral bone disorder (CKD–MBD) and confirm the changes in these genes and miRNA expression under uremic conditions by using a cell culture system. PubMed searches using MeSH terms and keywords related to CKD, uremia, and mRNA arrays were conducted. Through a computational analysis, a meta-signature that characterizes the significant intersection of differentially expressed mRNA and expected miRNAs associated with CKD–MBD was determined. Additionally, changes in gene and miRNA expressions under uremic conditions were confirmed with human Saos-2 osteoblast-like cells. A statistically significant mRNA meta-signature of upregulated and downregulated mRNA levels was identified. Furthermore, miRNA expression profiles were inferred, and computational analyses were performed with the imputed microRNA regulation based on weighted ranked expression and putative microRNA targets (IMRE) method to identify miRNAs associated with CKD occurrence. TLR4 and miR-146b levels were significantly associated with CKD–MBD. TLR4 levels were significantly downregulated, whereas pri-miR-146b and miR-146b were upregulated in the presence of uremic toxins in human Saos-2 osteoblast-like cells. Differentially expressed miRNAs associated with CKD-MBD were identified through a computational analysis, and changes in gene and miRNA expressions were confirmed with an in vitro cell culture system.
关键词: chronic kidney disease microRNA mineral bone disorder uremia
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 20-25 doi: 10.1007/s11684-009-0006-9
关键词: 4-1BB ligand tumor immunotherapy tumor microenvironment
Changlin Cao, Jingxian Gu, Jingyao Zhang
《医学前沿(英文)》 2017年 第11卷 第2期 页码 169-177 doi: 10.1007/s11684-017-0505-z
关键词: soluble triggering receptor expressed on myeloid cells-1 infectious diseases diagnosis and prognosis biomarker
Alternative splicing of inner-ear-expressed genes
null
《医学前沿(英文)》 2016年 第10卷 第3期 页码 250-257 doi: 10.1007/s11684-016-0454-y
Alternative splicing plays a fundamental role in the development and physiological function of the inner ear. Inner-ear-specific gene splicing is necessary to establish the identity and maintain the function of the inner ear. For example, exon 68 of Cadherin 23 (Cdh23) gene is subject to inner-ear-specific alternative splicing, and as a result, Cdh23(+68) is only expressed in inner ear hair cells. Alternative splicing along the tonotopic axis of the cochlea contributes to frequency tuning, particularly in lower vertebrates, such as chickens and turtles. Differential splicing of Kcnma1, which encodes for the α subunit of the Ca2+-activated K+ channel (BK channel), has been suggested to affect the channel gating properties and is important for frequency tuning. Consequently, deficits in alternative splicing have been shown to cause hearing loss, as we can observe in Bronx Waltzer (bv) mice and Sfswap mutant mice. Despite the advances in this field, the regulation of alternative splicing in the inner ear remains elusive. Further investigation is also needed to clarify the mechanism of hearing loss caused by alternative splicing deficits.
Ant colony optimization for assembly sequence planning based on parameters optimization
Zunpu HAN, Yong WANG, De TIAN
《机械工程前沿(英文)》 2021年 第16卷 第2期 页码 393-409 doi: 10.1007/s11465-020-0613-3
关键词: assembly sequence planning ant colony optimization symbiotic organisms search parameter optimization
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 97-109 doi: 10.1007/s11684-016-0496-1
As muscle activity during growth is considerably important for mandible quality and morphology, reducing dietary loading directly influences the development and metabolic activity of mandibular condylar cartilage (MCC). However, an overall investigation of changes in the protein composition of MCC has not been fully described in literature. To study the protein expression and putative signaling in vivo, we evaluated the structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat MCC at developmental stages. Isobaric tag for relative and absolute quantitation-based 2D nano-high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight/ time-of-flight (MALDI-TOF/TOF) technologies were used. Global protein profiling, KEGG and PANTHER pathways, and functional categories were analyzed. Consequently, histological and tartrate-resistant acid phosphatase staining indicated the altered histological structure of condylar cartilage and increased bone remodeling activity in hard-diet group. A total of 805 differentially expressed proteins were then identified. GO analysis revealed a significant number of proteins involved in the metabolic process, cellular process, biological regulation, localization, developmental process, and response to stimulus. KEGG pathway analysis also suggested that these proteins participated in various signaling pathways, including calcium signaling pathway, gap junction, ErbB signaling pathway, and mitogen-activated protein kinase signaling pathway. Collagen types I and II were further validated by immunohistochemical staining and Western blot analysis. Taken together, the present study provides an insight into the molecular mechanism of regulating condylar growth and remodeling induced by reducing dietary loading at the protein level.
关键词: condylar cartilage mechanical loading proteomic analysis iTRAQ bioinformatics analysis
Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells
Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 201-213 doi: 10.15302/J-FASE-2014017
关键词: repertoire complementarity determining region 3 of the IgH chain (CDRH3) immunoglobulin heavy chain variable region
莫茜,罗毅
《中国工程科学》 2008年 第10卷 第11期 页码 65-68
利用petri网直观图形化特点建立装配模型,并根据petri网原理得出可行装配序列。影响可行装配序列的大部分因素是定性的、模糊的、非数值的,将装配序列看作灰色系统,采用灰色聚类决策方法对可行序列进行评估。分析了影响因素的灰色分类并且研究了灰色聚类决策步骤。实例分析表明,该方法为petri网原理所得可行序列进行正确的评估并且得出决策向量。
Pankaj SHARMA,Ajai JAIN
《机械工程前沿(英文)》 2014年 第9卷 第4期 页码 380-389 doi: 10.1007/s11465-014-0315-9
Stochastic dynamic job shop scheduling problem with consideration of sequence-dependent setup times are among the most difficult classes of scheduling problems. This paper assesses the performance of nine dispatching rules in such shop from makespan, mean flow time, maximum flow time, mean tardiness, maximum tardiness, number of tardy jobs, total setups and mean setup time performance measures viewpoint. A discrete event simulation model of a stochastic dynamic job shop manufacturing system is developed for investigation purpose. Nine dispatching rules identified from literature are incorporated in the simulation model. The simulation experiments are conducted under due date tightness factor of 3, shop utilization percentage of 90 % and setup times less than processing times. Results indicate that shortest setup time (SIMSET) rule provides the best performance for mean flow time and number of tardy jobs measures. The job with similar setup and modified earliest due date (JMEDD) rule provides the best performance for makespan, maximum flow time, mean tardiness, maximum tardiness, total setups and mean setup time measures.
关键词: scheduling stochastic dynamic job shop sequence-dependent setup times dispatching rule simulation
null
《医学前沿(英文)》 2018年 第12卷 第1期 页码 23-33 doi: 10.1007/s11684-017-0607-7
Two decades have passed since the first bacterial whole-genome sequencing, which provides new opportunity for microbial genome. Consequently, considerable genetic diversity encoded by bacterial genomes and among the strains in the same species has been revealed. In recent years, genome sequencing techniques and bioinformatics have developed rapidly, which has resulted in transformation and expedited the application of strategy and methodology for bacterial genome comparison used in dissection of infectious disease epidemics. Bacterial whole-genome sequencing and bioinformatic computing allow genotyping to satisfy the requirements of epidemiological study in disease control. In this review, we outline the significance and summarize the roles of bacterial genome sequencing in the context of bacterial disease control and prevention. We discuss the applications of bacterial genome sequencing in outbreak detection, source tracing, transmission mode discovery, and new epidemic clone identification. Wide applications of genome sequencing and data sharing in infectious disease surveillance networks will considerably promote outbreak detection and early warning to prevent the dissemination of bacterial diseases.
关键词: genome sequencing genomic epidemiology bacteria surveillance infectious diseases
A novel sequence batch membrane carbonation photobioreactor developed for microalgae cultivation
Caiyun Hou, Sen Qiao, Yue Yang, Jiti Zhou
《环境科学与工程前沿(英文)》 2019年 第13卷 第6期 doi: 10.1007/s11783-019-1176-6
关键词: Membrane carbonation SBM-C-PBR Cultivate microalgae Membrane fouling
《医学前沿(英文)》 2023年 第17卷 第3期 页码 458-475 doi: 10.1007/s11684-022-0968-4
关键词: LU domain-containing protein family novel human gene LY6A pituitary tumor biomarker nonsynonymous SNP GPI-anchored protein
Assembly design system based on engineering connection
Wensheng YIN
《机械工程前沿(英文)》 2016年 第11卷 第4期 页码 423-432 doi: 10.1007/s11465-016-0382-1
An assembly design system is an important part of computer-aided design systems, which are important tools for realizing product concept design. The traditional assembly design system does not record the connection information of production on the engineering layer; consequently, the upstream design idea cannot be fully used in the downstream design. An assembly design model based on the relationship of engineering connection is presented. In this model, all nodes are divided into two categories: The component and the connection. Moreover, the product is constructed on the basis of the connection relationship of the components. The model is an And/Or graph and has the ability to record all assembly schemes. This model records only the connection information that has engineering application value in the product design. In addition, this model can significantly reduce the number of combinations, and is very favorable for the assembly sequence planning in the downstream. The system contains a connection knowledge system that can be mapped to the connection node, and the connection knowledge obtained in practice can be returned to the knowledge system. Finally, VC++6.0 is used to develop a prototype system called Connect-based Assembly Planning (CAP). The relationship between the CAP system and the commercial assembly design system is also established.
关键词: product design assembly design engineering connection assembly sequence planning
Data mining of microarray for differentially expressed genes in liver metastasis from gastric cancer
Ling XU MM, Feng WANG MM, Xuan-Fu XU MD, Wen-Hui MO BM, Rong WAN MD, Chuan-Yong GUO MD, Xing-Peng WANG MD,
《医学前沿(英文)》 2010年 第4卷 第2期 页码 247-253 doi: 10.1007/s11684-010-0027-4
关键词: gastric carcinoma metastasis signal transduction gene chips
标题 作者 时间 类型 操作
Annotation and validation of genes involved in photosynthesis and starch synthesis from a
Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG
期刊论文
Identification of differentially expressed miRNAs associated with chronic kidney disease–mineral bone
null
期刊论文
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
期刊论文
Soluble triggering receptor expressed on myeloid cell-1 (sTREM-1): a potential biomarker for the diagnosis
Changlin Cao, Jingxian Gu, Jingyao Zhang
期刊论文
Ant colony optimization for assembly sequence planning based on parameters optimization
Zunpu HAN, Yong WANG, De TIAN
期刊论文
iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar
null
期刊论文
Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells
Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO
期刊论文
Analysis of dispatching rules in a stochastic dynamic job shop manufacturing system with sequence-dependent
Pankaj SHARMA,Ajai JAIN
期刊论文
Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the
null
期刊论文
A novel sequence batch membrane carbonation photobioreactor developed for microalgae cultivation
Caiyun Hou, Sen Qiao, Yue Yang, Jiti Zhou
期刊论文
novel human gene, LY6A, which encodes the candidate ortholog of mouse Ly-6A/Sca-1 and is aberrantly expressed
期刊论文